📰 A new way to assess the fatty acid composition of fish

📰 A new way to assess the fatty acid composition of fish

In collaboration with SYSAAF, the company Aqualande and INRAE, scientists – CNRS / University of Rennes 1 – of the ScanMAT Support and Research Unit, the Rennes Institute of Chemical Sciences and the Rennes Institute of Physics they propose an alternative method using micro-Raman. spectroscopy to assess composition fatty acid (In chemistry, a fatty acid is an aliphatic carboxylic acid. It is …) adipose tissue of fish. Less expensive, non-destructive, and faster than current baseline analyzes, it allowed them to reliably measure the omega-3s present in the tissues of the trout (The term trout is an ambiguous vernacular name that designates several species of fish …) Rainbow (A rainbow is an optical and meteorological phenomenon that makes visible the …). This method could be used for selection genetics (Genetics (from the Greek genno γεννώ = to give birth) is …) of farmed fish by predicting their fatty acid composition beneficial to the Health (Health is a state of complete physical, mental and social well-being, and it is not …).


It predicts the fatty acid composition of rainbow trout at low cost by Raman miscospectroscopy.
© E. Prado

the fish (Pisces is a constellation of the zodiac crossed by the Sun from March 12 to 18 …) is the main source of long-lasting polyunsaturated fatty acids Chain (The word string can have several meanings 🙂 omega 3 in the human diet, the beneficial effects of which on health are known. We are currently using the chromatography (Chromatography is a technique for separating analytical chemistry that can be …) en phase (The word phase can have several meanings, it is used in several fields and …) gas for the identification and quantification of these fatty acids. But its high cost and duration of analyzes make it ineffective in phenotyping a broadband (The term broadband (or broadband by literal translation of the Anglo-Saxon expression …) necessary for genetic selection programs of cultivated aquaculture species.

Scientists from SYSAAF and the CNRS / University of Rennes 1 of Support and Research (Scientific research designates in the first place all the actions carried out with the aim of …) ScanMAT, elhigh school (An institute is a permanent organization created for a specific purpose. It is …) chemical sciences of Rennes and the Institute of Physics (Physics (from the Greek φυσις, nature) is etymologically the …) of Rennes propose, therefore, to use Raman microspectroscopy which they have specifically adapted for the identification of these biological molecules. A non-destructive, fast and economical method, they prove to be especially reliable for recognizing and quantifying these fatty acids. For this reason and in order to obtain an important variability of the composition in acid (An acid is a chemical compound usually defined by its reactions …) Tissue fat, 268 rainbow omelettes, subjects of the study, were fed various foods with very different fatty acid compositions, which went as far as one food. vegetable (The group of classical scientific classifications under the term plant …) which is totally missing. Comparison of the composition evaluated by chromatography (CPG) with the result of the analysis of the Raman spectra showed a good reliability (A system is reliable when the probability of fulfilling its mission over a period of time …) of this method to detect total polyunsaturated fatty acids, omega 6 and omega 3 (EPA, DHA) present in the tissue shown *.

This study, which shows that Raman spectroscopy can be used to predict the fatty acid composition of rainbow trout adipose tissue, could now allow its phenotyping to be considered at a high level. debit (A flow allows you to measure the flow of a quantity relative to a unit of time through …) necessary for genetic selection programs. The results will be found in the review Analytica Chemicala Acta.

Note:
* In fish, lipids accumulate in the liver (The liver is a strange and asymmetrical abdominal organ, housed in humans in …), muscles, periviceral and subcutaneous adipose tissue. the adipose tissue (Adipose tissue (too fat)) In this study, periviceral was chosen because its composition in fatty acids is close to that ofanimal (An animal (from the Latin animus, spirit or vital principle) is, according to the classical classification, a …) whole and this fabric is available in quantity (Quantity is a generic term for metrology (count, amount); a scalar, …) sufficient to perform chromatographic analysis. In addition, it is a fairly homogeneous tissue by which it is contaminated by muscle proteins or blood (Blood is a liquid connective tissue made up of populations of free cells, including …) they are limited.

These works are the fruit (In botany, the fruit is the plant organ that protects the seed ….) a collaboration between SYSAAF, ScanMAT, ISCR, IPR, INRAE ​​(AgroParisTech, Univ Pau & Country (Country comes from the Latin pagus which designated a territorial and tribal subdivision of extension …) Adour, PEIMA, Univ. de Rennes 1), IRMAR (Agrocampus West (The west is a cardinal point, opposite the east. This is the direction to …)Rennes) and the company Les Sources de l’Avance (Aqualande Group, Roquefort) as part of the project (A project is an irreversible commitment of uncertain outcome, not reproducible in …) OMEGA TRUITE (European Maritime and Fisheries Fund, n ° PFEA47 0017 FA 1000008).

Reference:
Prado, E .; Ekloh-Molinier, C .; Enez, F .; Parlador, D .; Blay, C .; Dupont-Nivet, M .; Labbe, L .; Petit, V .; Moreac, A .; Taupier, G .; Haffray, P .; Bugeon, J .; Corraze, G .; Nazabal, V.
Prediction of fatty acid composition in rainbow trout Oncorhynchus mykiss by Raman microscopy
Analytica Chemicala Acta 2022
http://dx.doi.org/10.1016/j.aca.2021.339212

Contact:
– Virginie Nazabal – Researcher at the Rennes Institute of Chemical Sciences – virginie.nazabal at univ-rennes1.fr
– Stéphanie Younès – Manager Communication (Communication affects both men (intrapsychic, interpersonal communication, etc.) – Institute of chemistry (Chemistry is a natural science divided into several specialties, for …) from CNRS (The National Center for Scientific Research, better known by its acronym CNRS, is the largest …) – inc.communication a cnrs.fr
– Anne-Valérie Ruzette – Manager scientific (A scientist is a person who is engaged in the study of one or more sciences and who …) for communication – CNRS Institute of Chemistry – anne-valerie.ruzette at cnrs.fr
says Christophe Cartier Mill (A mill is a machine for grinding cereal grains into flour and, by analogy, …)Search engine (A researcher (fem. Researcher) refers to a person whose job is to do research …) at the Institute of Molecular Chemistry in Paris and director of scientific communication projects at the INC
– inc.communication a cnrs.fr

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